<i>In vitro</i> host range of feline morbillivirus

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In vitro host range of feline morbillivirus

Feline morbillivirus (FmoPV) is an emerging virus in cats, which is associated with tubulointerstitial nephritis. To study the in vitro host range of FmoPV, we inoculated FmoPV strain SS1 to 32 cell lines originated from 13 species and cultured for 2 weeks, followed by RNA extraction and reverse-transcription-polymerase chain reaction for FmoPV detection. As a result, only cell lines derived fr...

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Basic biological characterization of feline morbillivirus

Feline morbillivirus (FmoPV) is an emerging virus that was recently discovered in domestic cats with chronic nephritis. Despite the potential role of FmoPV in chronic nephritis, little is known about its biological characteristics. In this study, we established a quantitative assay of FmoPV by using an indirect immunofluorescence technique. Viral titers of FmoPV were determined in one week. Tre...

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Chronic Infection of Domestic Cats with Feline Morbillivirus, United States.

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Quantitative PCR detection of feline morbillivirus in cat urine samples

Feline morbillivirus (FmoPV) is a new virus species and its detection is important, since correlation has been reported between FmoPV virus infection and tubulointerstitial nephritis in cats. Here, we report a real-time reverse transcription (RT)-PCR system that can detect the FmoPV L-gene sequence with more than 10-time higher sensitivity than a conventional PCR system, resulting in detection ...

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ژورنال

عنوان ژورنال: Journal of Veterinary Medical Science

سال: 2015

ISSN: 0916-7250,1347-7439

DOI: 10.1292/jvms.15-0213